Cave Microbiomes: A Novel Resource for Drug Discovery by Naowarat Cheeptham (auth.), Naowarat Cheeptham (eds.)

By Naowarat Cheeptham (auth.), Naowarat Cheeptham (eds.)

This ebook info contemporary findings within the box of cave microbiology and builds on fast paced efforts to take advantage of an unconventional and underexplored atmosphere for brand new microorganisms that may supply an untapped resource of substances: microorganisms from caves.

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Recently, in situ cell disruption methods including both physical and chemical protocols which include a subset of grinding–freezing–thawing, sonication, beadbeating, heating treatment, and/or detergent have been adapted for nucleic acid purification (Robe et al. 2003). Studies comparing procedures for efficient DNA extraction from different geological samples showed different levels of efficiency and quality of DNA recovered. In summary, it was demonstrated that depending on unique characteristics of each sample, different DNA extraction procedures and steps involved in such endeavors needed to be tested to evaluate which procedure was the most appropriate to maximize extract purification (Herrera and Cockell 2007).

Tufas and speleothems: unravelling the microbial and physical controls. University of Alberta, p 9–10. Jurado V, Groth I, Gonzalez JM et al (2005) Agromyces subbeticus sp. , isolated from a cave in southern Spain. Int J Syst Evol Microbiol 55:1897–1901 Jurado V, Gonzalez JM, Laiz L et al (2006) Aurantimonas altamirensis sp. , a member of the order Rhizobiales isolated from altamira cave. Int J Syst Evol Microbiol 56:2583–2585 Kirk JL, Beaudette LA, Hart M et al (2004) Methods of studying soil microbial diversity.

1. These colorful features are natural biofilms known as bacterial mats. Biofilms are cell populations that irreversibly adhere to solid, biotic, or abiotic surfaces and that are surrounded by an exopolysaccharide (EPS) matrix, as illustrated by Fig. 2. In biofilms, microbial cells live in structured, coordinated, functional communities and are able to thrive more efficiently than planktonic cells in many natural environments (O’Toole et al. 2000). Biofilm formation starts with the attachment and immobilization of free-living (planktonic) cells, the primary colonizers, to a surface.

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