Cell Engineering: Transient Expression by T. A. Kost, J. L. Klein, J. P. Condreay (auth.), Mohamed

By T. A. Kost, J. L. Klein, J. P. Condreay (auth.), Mohamed Al-Rubeai (eds.)

The benefits of the baculovirus process are rooted within the houses of the virus and the host (insect, or cellphone traces derived from it). through the general an infection cycle, sorts of the virus are produced: an early budded virus (BY) shape (Kost et al. , 2000), within which the viral DNA and structural proteins are surrounded via membrane derived from the contaminated mobilephone; and a past due occluded shape (occlusion-derived virus, ODy), including enveloped viral cores that are embedded in a crystal matrix of viral proteins. The valuable section of the matrix is the abundantly expressed protein polyhedrin. The budded virus speedily spreads the an infection from cellphone to mobilephone in the insect host, ensuing eventually within the whole liquefaction of the host, and free up of occluded virus into the surroundings. The occluded shape protects the published virus, permitting it to outlive for lengthy classes within the atmosphere until eventually ingested through one other host. within the alkaline surroundings ofthe insect intestine, the protecting protein matrix is got rid of, and the existence cycle is repeated. In insect mobilephone cultures, basically the BV kind of baculovirus is needed, and the polyhedrin gene can be changed with the gene for the recombinant protein. an extra good thing about exchanging or deleting polyhedrin is that it successfully makes the virus not able to outlive open air the laboratory, a bonus when it comes to environmental protection. The process is intrinsically secure to animals, being not able to copy in species except a constrained diversity of insects.

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1998) Steroid hydroxylation by human fetal CYP3A7 and human NADPH-cytochrome P450 reductase coexpressed in insect cells using baculovirus. Res. Commun. Mol. Path. Pharmacol. 100,15-28. , Miners, J. , Birkett, D. , and Bhrasker, C. R. (1998) Baculovirus-mediated expression of cytochrome P4502C8 and human NADPH-cytochrome P450 reductase: optimization of protein expression. Xenobiotica 28,137-152. W. (1995) The baculovirus gp64 envelope fusion protein: synthesis, oligomerization, and processing. Virology 209, 592-603.

Prot. Expr. Purif. 10,325-330. , and Rei Hinder, H. (2000) Engineering of a proteolytically stable human pz-adrenergic receptor/maltose-binding protein fusion and production of the chimeric protein in Escherichia coli and baculovirus-infected insect cells. J. Biotechnol. 77,219-234. Y. (1991) Insect virus: assays for viral replication and persistence in mammalian cells. J. Viral. Meth. 31,335-344. , and Strauss, M. (1999) Protection of baculovirus-vectors against complement-mediated inactivation by recombinant soluble complement receptor type 1.

Though these processing events occur in insect cells, the modifications are not always identical to those that occur in mammalian cells. For certain applications these differences may prove to be extremely important. 29 M. ), Cell Engineering, 29-42. © 2000 Kluwer Academic Publishers. 30 Many post-translational processing events occur within the secretory pathway. The secretory pathway is a multi-organelle system that transports polypeptides through multiple compartments where they are subsequently cleaved, folded, modified, assembled, and secreted or displayed at the membrane surface.

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