Disney's Bonkers Joke Book (Golden Books) by Linda Williams Aber, Darrell Baker

By Linda Williams Aber, Darrell Baker

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1968). Cross reaction between these two lectins was excluded by the doubleincubation technique. Moreover, the receptor for PNA is destructible by chymotrypsin, the receptor of RCA by trypsin (Vierbuchen et al. 1980; Klein et al. 1980). The specificity of the lectin binding was proved by inhibition with D-galactose and Blactose for RCA and with asialoglycophorin from human erythrocytes, which contains large amounts of gal-B(1-3) NAcgal (Thomas and Winzler 1969). The experimental details have been described previously (Klein et al.

Histochemical procedure: 1. Incubation of the tissue section with neuraminidase (10 - 20 mU/tissue 'section) for 30 min in a moist chamber at 37 0 C 2. 15 M sodium chloride) 3. Incubation with the labeled lectin (10-20 ~g/tissue section) 4. Repeated washing with PBS 5. Examination of the tissue section under fluorescence microscope /Zeiss epifluorescence 435-490 nm and 546-590 nm spectrum filters, HBO 50W mercury lamp) The immunohistologic studies with labeled antibodies were performed on frozen tissue sections.

Case Reports Our first patient in one family was a 2-year-old boy with mild proteinuria and permanent microscopic hematuria. His mothers' disease was diagnosed as mesangioproliferative glomerulonephritis when she was 28. His uncle and grandmother died of uremia. His elder sisters are healthy. Both mother and son have a defect in audiometry. Electron microscopy of a renal biopsy showed that the basement membrane had segmentary narrowing with characteristic splitting in multiple thin layers, separated by clear spaces, containing dense particles (Dept.

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