Invertebrate Tissue Culture: Research Applications by Karl Maramorosch

By Karl Maramorosch

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BUTTERFLY CELL LINES 560 chromosomes were counted in one metaphase plate. Since the Px 58 cell did not respond well to colchicine treatment, and only limited numbers of metaphase plates were obtained, no statistical analysis of the karyotype has been made. D. GROWTH The growth curves of the Px 58 cell are shown in Fig. 11. From this figure the population doubling time at 25°C was estimated to be about 60 hours in MGM-431 medium. If the medium was not renewed, the multiplication rate of the cell decreased usually 1 week after the subculture.

1970), for instance, the first subculturing was done 3 weeks after the culture was set up. The long lag period for the cell growth in the primary culture, therefore, may be limited to the lepidopteran ovarian tissue culture. This long lag period, however, may be shortened if the culture medium is improved. The Px 58 cell line has had more than 300 passages, and the Px 64 2. BUTTERFLY CELL LINES 35 cell line already more than 150 passages. In mammalian cell cultures, especially human cell cultures, it is recognized that the cell often is fated to die at around the fiftieth passage.

In the media lacking other amino acids, the cell deteriorated sooner or later. The cell, however, could adapt to the medium lacking serine. In this medium the cell stayed alive for a long time and gradually multiplied; finally, the growth rate of the cell was comparable to that of the control. In the medium lacking glutamine, the cell attached itself to the glass and stayed alive for several months, but apparently did not multiply. The utilization and requirement of amino acids by the Px 58 cell are summarized as follows.

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