Methods of Biochemical Analysis, Volume 10 (v. 10) by David Glick

By David Glick

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L. A. , Vapour Phase Chromatography, Butterworths, London, 1957. 13. Gunner, S. , J. K. N. JoneA, and M. B. , London, 1961, 255. 14. Hamilton, J. , and F. Smith, J. Am. Chem. , 78,5907 (1956). 15. , W. Nel, and V. Pretorius, Nature, 181,177 (1958). 16. Haworth, W. , J. Chem. , 107,8 (1915). 42 C. T. BISHOP 17. Hedgley, E. , 0. Meresz, W. G. Overend, and R. Rennie, Chem. & Znd.. London,1960, 938. 18. Hedgley, E. , and W. G. Overend, Chem. , London,1960,378. 19. , Methods of Biochem. , 1,205 (1954).

Separation of the fully acetylated monosaccharides (23) may offer better possibilities because it may be that one ring form will predominate under acetylation conditions and mixtures of anomeric acetates can be isomerized to a single anomer. Separation of fully acetylated glycitols has been used for the quantitative analysis of mixtures of monosaccharides (13). The monosaccharides were reduced by sodium borohydride, and the mixture of glycitols was then acetylated by acetic anhydride in the presence of sulfuric acid.

C. Alkylmercuric Compounds. . . . . . . . . . . . . . . 5. -SH Assay with Organic Mercury Compounds. . . . . . . . sing Nitroprusside aa I .................... xcess Mercurial. . . . . ............. IV. Alkylation . . . . . . 1. Determination of S H groups as S-Carboxymethylcysteie... . 2. Determination of Liberated Hydrogen Ions.. . . . . . . V. Addition to Double Bonds. . . . . . , . . . . . . . . . . . . . VI. Conclusion. . .

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