Research Surgery and Care of the Research Animal: Surgical by William Gay, William I. Gay, James E. Heavner

By William Gay, William I. Gay, James E. Heavner

Equipment of Animal Experimentation, quantity VII: learn surgical procedure and Care of the examine Animal, half C is a suite of papers that bargains with equipment utilized in animal experiments referring to surgical techniques to convinced organ platforms reminiscent of the maxillofacial and dermis platforms. This assortment offers with surgical procedure regarding the oral and maxillofacial, ophthalmic, dermis platforms, in addition to learn on dogs immune structures and purposes of microsurgery and laser surgical procedure. One paper discusses orthognathic surgical procedure together with temporomandibular joint and dental implants. one other paper describes corneal approaches, lens extraction, and intraocular lenses. In discussing pores and skin experimental surgical procedure, the writer explains techniques in flap anatomy, surgical therapeutic, epidermis grafting, and burn therapy. The innovations in learning the immune structures of canine contain systems facing peripheral blood, bone marrow, reactive cells, and tissues. different authors clarify the applying of microsurgery to laboratory study together with the apparatus and methods used, in addition to the overall ideas of tissue transplantation into the animal's crucial worried approach. one other writer discusses using lasers and precise concerns comparable to laser regulations and instrumentation. This ebook could be liked by way of laboratory assistants and scientists facing try out animals, by way of veterinarians, and by way of researchers designing animal and scientific experiments.

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H e m o s t a s i s should be applied using 2-0 silk. T h e p e r i c a r d i u m c a n n o w b e easily r e t r a c t e d with Allis or B a b c o c k forceps to e x p o s e an a r e a desired or tied with 2-0 silk and clamped to the surgical t o w e l s to form a pericardial cradle. B . Midline Thoracotomy A midline sternal incision is preferred in the p r i m a t e , especially the b a b o o n a n d c h i m p a n z e e w h e r e the chest is very d e e p . This a p p r o a c h allows the p l e u r a to remain intact for better closure and e n h a n c e s the time of r e c o v e r y .

T h e skin is closed with 3-0 silk on a straight cutting needle using an interrupted horizontal m a t t r e s s suture p a t t e r n . A 2- to 3-cm tag should be left on the skin suture for easy r e m o v a l in 10 d a y s to 2 w e e k s . T h e lead wires, having b e e n brought CARDIOVASCULAR SURGERY IN CONSCIOUS ANIMALS 45 through the skin at the m i d c h e s t level, are run subcutaneously to the interscapular region and again externalized. T h e catheters are filled with heparinized saline (20 U/ml) and sealed by placing a knot in the end of the c a t h e t e r .

Surgical Procedure T h e animal is placed supine. A t r a c h e o s t o m y is performed after which a median s t e r n o t o m y skin incision is m a d e and the sternum and anterior ribs e x p o s e d . B e c a u s e the animals are usually small, the anterior rib cage is usually r e m o v e d by severing through the ribs on both sides and r e m o v ing the s t e r n u m and anterior ribs together. Animals are continuously mechanically ventilated during this p r o c e s s . V e n o u s access is obtained, usually via a v e n o u s c u t d o w n and the animal is heparinized.

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